- 12 – Route 2 east of Route 110/111
- 14 – Route 2 west of Route 110/111
- 21 – Out of town assist
- 31 – Old Library
- 32 – Harvard Common
- 33 – Unitarian Church
- 34 – Town Hall
- 35 – Fire Station
- 36 – Congregational Church
- 41 – Public Safety Building
- 45 – Still River Fire Station
- 52 – Town Beach
- 53 – Lancaster Shore, Turners Lane
- 54 – Willard Lane, Clinton Shore Drive
- 112 – St. Theresa Catholic Church
- 113 – Willow Road
- 114 – Madigan Lane
- 115 – Still River Road from town center to Prospect Hill Road
- 116 – Still River Depot Road
- 117 – Still River Road at Bolton town line, St. Benedict’s School
- 118 – Under Pin Hill Road, Lovers Lane
- 121 – Depot Road at Craggs Road, Pattee Road, Transfer Station
- 122 – Harvard Depot Road
- 123 – Prospect Hill Road, Fruitlands
- 124 – Whitney Road
- 125 – Mill Road
- 126 – Old Shirley Road
- 131 – Ayer Road from town center to Route 2
- 132 – Ayer Road from Route 2 to Old Mill Road
- 133 – Ayer Road from Old Mill Road to Ayer town line, Appleworks Building
- 134 – Lancaster County Road, Cedar Ledge Road
- 135 – Old Mill Road
- 136 – Blanchard Road
- 141 – Poor Farm Road
- 142 – South Shaker Road, Quarry Lane
- 143 – Glenview Drive
- 144 – South Shaker Road at Ayer town line, Sheehan Road
- 145 – Shaker Hills
- 211 – Littleton Road from Fairbank Street to Cruft Lane, Orchard Hill
- 212 – Littleton Road from Cruft Lane to Route 2 bridge
- 213 – Littleton Road from Route 2 bridge to Littleton town line
- 214 – Sawyer Lane
- 215 – Old Littleton Road from Fairbank Street to Old Schoolhouse Road
- 216 – Old Littleton Road from Old Scholhouse Road to Littleton town line
- 217 – Green Hill Road
- 221 – Pinnacle Road
- 222 – Park Lane
- 223 – Little Common (Fairbanks Street)
- 224 – Oak Hill Road
- 225 – Cleaves Hill Road
- 226 – Woodchuck Hill Road
- 227 – Route 111 at Stow Road
- 231 – Route 111 at Boxborough town line
- 232 – Sherry Lane
- 233 – Littleton County Road at Cleaves Hill Road
- 234 – Littleton County Road at Littleton town line
- 235 – Slough Road
- 236 – Stow Road from Mass Avenue to Codman Hill Road
- 241 – Stow Road from Codman Hill Road to town line
- 242 – Murray Lane
- 243 – Eldridge Road
- 311 – Finn Road at Stow town line
- 312 – Hillcrest Drive
- 313 – Withington Lane
- 314 – East Bare Hill Road
- 315 – Westcott Road
- 316 – Jacob Gates Road
- 321 – Bromfield Street at Bolton Road
- 322 – Bolton Road at West Bare Hill Road
- 323 – Bolton Road at Bolton town line
- 324 – Partridge Hill Road, Deerfoot Trail
- 325 – Brown Road
- 411 – West Bare Hill Road
- 412 – Woodside Road
- 413 – Scott Road, Candleberry Lane
- 414 – Bowers Road
- 511 – Warren Avenue
- 512 – Tahanto Trail
- 513 – Peninsula Road
- 4321 – Elementary School
- 5321 – New Library
- 3321 – Bromfield House
- 6321 – The Bromfield School
Special Signals
- 10 – repeated – Civil Defense call
- 2-2-2 – all clear
- 5-5-5 – no school, blown at 7:00 a.m.
- 2 – test blows at 12:00 noon